Enzymes in Metabolism of Amino Acids. VII.* Phenylalanine Ammonia-Lyase in Maize {Zea mays L.)

نویسندگان

  • P. NEMEC
  • M. PŠENÁK
  • P. KOVÁCS
چکیده

We have isolated an enzyme, phenylalanine ammonia-lyase (EC 4.3.1.5.), from the rootlets of Zea mays var. СЕ-IV. After partial purification by fractional salting out with ammonium sulfate and gel chromatography on Sephadex G-100, we obtained a preparation of a 40-fold greater specific activity than that of the crude homogenate. We hbve determined the ap­ parent Michaelis constant and the activation energy of this purified pre­ paration and investigated the effect of some phenolic compounds on its activity.

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تاریخ انتشار 2013